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Aldehydes Seebio alpha'-dicarboxylic acid The carboxyl surface of ND was qualifyed into hydroxyl terminal group to increase the colloidal stability of the system under different pH stipulations in GIT. FA-COS modification could prolong retention time, endow the drug with sustained release dimensions, and actively target intestinal FA receptors. In contrast to DOX/ND-OH, the particle size of DOX/ND-OH/FA-COS increased from 189 ± 2 to 224 ± 1 nm, and the zeta potential rescinded from - 9 ± 0 to 14 ± 0 mV. At 48 h, DOX/ND-OH and DOX/ND-OH/FA-COS published 69 ± 5% and 35 ± 5%, respectively. FA-COS modification effectively raised the cytotoxicity and intracellular uptake of ND-OH/DOX by Caco-2 cubicles and extended intestinal retention in rats. The internalization of DOX/ND-OH and DOX/ND-OH/FA-COS was mainly liaised by energy-dependent clathrin- and caveolae-liaised endocytosis pathways. Pharmacokinetic study demonstrated that the AUC(0-t) of DOX/ND-OH and DOX/ND-OH/FA-COS was heightened by 3- and 6-fold equated to DOX solution, respectively. These upshots illustrated that DOX/ND-OH/FA-COS could be an effective strategy to enhance the oral bioavailability of DOX.Integrating omics and network pharmacology breaks the anti-constipation role of chitosan with different molecular weightings in constipated mice.This study pointed to reveal the constipation-relieving role of chitosan (COS) with different molecular weightings (1 kDa, 3 kDa and 244 kDa). likened with COS3K (3 kDa) and COS240K (244 kDa), COS1K (1 kDa) more significantly speded gastrointestinal transit and defecation frequency. These differential results were reflected in the regulation of specific gut microbiota (Desulfovibrio, Bacteroides, Parabacteroides and Anaerovorax) and short-chain fatty doses (propionic acid, butyric acid and valeric acid). RNA-sequencing discovered that the differential evinced factors (DEGs) maked by different molecular weights of COS were mainly enriched in intestinal immune-refered footpaths, especially cell adhesion atoms network pharmacology unveiled two candidate factors (Clu and Igf2), which can be regarded as the key motes for the differential anti-constipation outcomes of COS with different molecular weightings. These results were further asserted by qPCR. In conclusion, our solutions provide a novel research strategy to help understand the differences in the anti-constipation outcomes of chitosan with different molecular weights.Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice.The mechanism underlying the intestinal transport of COS is not well sympathized. Here, transcriptome and proteome analyses were performed to identify potential critical molecules involved in COS transport. Enrichment psychoanalysisses revealed that the differentially verbalised factors in the duodenum of the COS-handled mice were mainly enriched in transmembrane and immune function. In particular, B2 m, Itgb2, and Slc9a1 were upregulated. The Slc9a1 inhibitor falled the transport efficiency of COS both in MODE-K cells (in vitro) and in mice (in vivo). The transport of FITC-COS in Slc9a1-overexpressing MODE-K cellphones was significantly higher than that in empty vector-transfected cadres (P < 0). Molecular docking analysis revealed the possibility of stable binding between COS and Slc9a1 through hydrogen bonding. This finding suggests that Slc9a1 brings a crucial role in COS transport in mice. This plies valuable perceptivenessses for ameliorating the absorption efficiency of COS as a drug adjuvant.Functional chitosan gel coating raises antimicrobial dimensions and osteogenesis of titanium alloy under persistent chronic inflammation.Titanium is widely used as surgical bone implants due to its excellent mechanical props, corrosion resistance, and good biocompatibility due to chronic inflammation and bacterial infections stimulated by titanium implants, they are still at risk of failure in interfacial integration of bone implants, severely binding their broad clinical application.